ABSTRACT
Background and Aim: The aim of this study was to investigate the protective effects of Tanacetum parthenium extract [TPE] on lipid peroxidation and acute heart injury induced by carbon tetrachloride [CCl4] in rats
Materials andmethods: In an experimental study, 30 male Wistar rats were divided into 5 groups [n=6]. Two of the groups were normal and damage control groups, other 3 groups were damage groups treated with 40, 80 and 120 mg / kg of TPE for 14 days before being damaged by CCl4. At the end of the study we measured the activity of creatine kinase [CK-MB] isoenzyme and lactate dehydrogenase [LDH], total cholesterol [TC], HDL-C and LDL-C in serum, as well as the activity of superoxide dismutase [SOD], glutathione peroxidase [GPX], and arylesterase [ARE] and malondialdehyde [MDA] concentration, as lipid peroxidation index, in myocardial homogenates
Results: The results of this study showed that treatment with carbon tetrachloride significantly increased the levels of CK-MB, LDH, TC, LDL-C and MDA and decreased ARE, HDL-C, SOD and GPX [P <0.001] in a dose dependent manner
Conclusion: The present study demonstrated that TPE can be considered as a substance with various potential effects such as antioxidant activity and cardioprotective effects
ABSTRACT
Gastric cancer as the fourth most frequent malignancy worldwide was known to have the highest rate among cancer-related disorders in Ardabil province. The product of TP53 gene regulated the cell cycle process and acts as a tumor suppressor factor. The polymorphism P53 Arg72Pro [rs1042522] has been reported to be associated with many type of cancers. The purpose of the present study was investigating about susceptibility of gaining gastric cancer in which probably conferred by the polymorphism P53 Arg72Pro in Ardabil province. Using PCR-RFLP, the polymorphism was assayed among 87 patients affected with gastric cancer and 92 healthy controls selected. The statistical significance was analyzed by logistic regression test. The mean of age for cases and controls were 64.2 and 61.9 years respectively. The frequency of genotypes for cases has been detected as 16.3% for Pro/Pro, 41.9% for Arg/Pro, and 41.9% for Arg/Arg and for controls were 18.5% Pro/Pro, 40.2% Arg/Pro, and 41.3% Arg/Arg respectively. There was not any significant association between this polymorphism and affecting to gastric cancer. Finding shows relationship between susceptibility of gaining gastric cancer in our province and the polymorphism rsl 042522 could be due to genetic and population relationship between Ardabil population and Caucasians, and the selective advantage of the Arg allele in cold climates, as well. On the other hand, considering to the previous studies on the etiology of gastric cancer in Ardabil province shows, environmental factors such as nitrate have more important role bin conferring susceptibility to gain gastric cancer and some genetic changes and affecting to gastric cancer in this study indicates that the role of lifestyle improvement might reduce the huge rate of affected patients in our province
ABSTRACT
AIM@#The most important side effect of methotrexate (MTX) is mucositis. The purpose of this study was to evaluate the effect of turmeric extract on intestinal damage and oxidative stress in rats receiving methotrexate.@*METHODS@#Experiments were performed on male Wistar albino rats divided into six groups. First group received normal saline orally, the second group received turmeric extract (100 mg·kg(-1)) orally for 30 days, the third group received turmeric extract (200 mg·kg(-1)) orally for 30 days, the fourth group received a single dose of methotrexate (20 mg·kg(-1)) i.p. at day 30, the fifth group received turmeric extract (100 mg·kg(-1)) orally for 30 days and a single dose of methotrexate (20 mg·kg(-1)) i.p. at day 30, and the sixth group received turmeric extract (200 mg·kg(-1)) orally for 30 days and single dose of methotrexate (20 mg·kg(-1)) i.p. at day 30. Four days after methotrexate injection, animals were anesthetized, blood samples were taken to determine total antioxidant status (TAS) and jejunum samples were taken for glutathione peroxidase (GPx), superoxidase dismutase (SOD), catalase (CAT), aldehyde malondialdehyde (MDA), and histopathological assessment.@*RESULTS@#Microscopic evaluation from intestinal tissues of the MTX treated group, showed severe villus shortening and blunting, inflammatory cell infiltration and hemorrhage in lamina propria, along with epithlial cell necrosis. Levels of SOD, GSH-Px and CAT decreased in the MTX received group, but increased significantly (P < 0.05) in the turmeric + MTX groups. MTX increased lipid peroxidation, however, turmeric decreased peroxidation significantly (P < 0.05).@*CONCLUSION@#These results suggest that turmeric extract may protect the small intestine of rats from methotrexate-induced damage. Turmeric effects could result from its antioxidant properties.
Subject(s)
Animals , Humans , Male , Rats , Catalase , Metabolism , Curcuma , Chemistry , Glutathione Peroxidase , Metabolism , Intestinal Diseases , Drug Therapy , Metabolism , Intestinal Mucosa , Metabolism , Malondialdehyde , Metabolism , Methotrexate , Oxidative Stress , Plant Extracts , Rats, Wistar , Superoxide Dismutase , MetabolismABSTRACT
To determine the diagnostic value of the lactate concentration in vaginal secretions in determining the premature rupture of membranes. One hundred 100 pregnant women with single pregnancy of gestational age 20-41 weeks were divided to two groups of 50 each. One group comprised of women with premature rupture of membranes, and the other [control group] of women with intact membranes. To verify the premature rupture of membranes in both groups, the speculum, fern, and nitrazine tests were done. Lactate Pro manual instrument was used to measure the lactate levels in vaginal fluid via enzymatic staining which was displayed on the instrument's liquid crystal display after 60 seconds. Descriptive analytic statistics and SPSS 17 software were used to analyze the data. The lactate concentration of 4.6 mM in the vaginal fluid was diagnostic for premature rupture of membranes. It had a sensitivity of 96%, specificity of 98.8%, accuracy of 97%, positive predictive value of 97.9%, and negative predictive value of 96%. Testing lactate levels in the vaginal fluid is an easy, rapid and reliable method for the diagnosis of premature rupture of membranes in pregnancy